Under ideal conditions, the bacteria double every 20 minutes.
In 6 hours, at a temperature of 37 °C, an initial population of 100 bacteria can replicate up to a value higher than 26 million.

The determination of the antibacterial activity was performed according ISO 27447:2009, which is intended for use with different kinds of semiconducting photocatalytic materials used in construction materials, in flat sheet, board, plate shape or textiles that are the basic forms of materials for various applications.
This test method is usually applicable to photocatalytic materials produced for an antibacte-rial effect. Microbiological tests were performed by Biochemie Lab.

The study virucidal activity was carried out at the Department of Hygiene and Public Health, University of Florence (laboratory affluent to the regional network of reference laboratories, accredited to the virological monitoring and validated by the National Centre for WHO of the ISS-Department of Infectious Diseases.